منابع مشابه
Quantification of human plasma phospholipid transfer protein (PLTP): relationship between PLTP mass and phospholipid transfer activity.
A sensitive sandwich-type enzyme-linked immunosorbent assay (ELISA) for human plasma phospholipid transfer protein (PLTP) has been developed using a monoclonal capture antibody and a polyclonal detection antibody. The ELISA allows for the accurate quantification of PLTP in the range of 25-250 ng PLTP/assay. Using the ELISA, the mean plasma PLTP concentration in a Finnish population sample (n = ...
متن کاملSeparation of a plasma phospholipid transfer protein from cholesterol ester/phospholipid exchange protein.
The d > 1.21 g/ml fraction of human plasma stimulates mass transfer of phosphatidylcholine (PC) from egg PC vesicles into plasma high density lipoproteins (HDL) and also exchange of PC and cholesterol esters (CE) between HDL and low density lipoproteins (LDL). By measuring the facilitated transfer of PC from egg PC vesicles into HDL, and the facilitated exchange of cholesterol esters between HD...
متن کاملBiosynthesis and secretion of human plasma phospholipid transfer protein.
Plasma phospholipid transfer protein (PLTP) plays a critical role in lipoprotein metabolism and reverse cholesterol transport. We have studied the biosynthesis and secretion of PLTP using a stably transfected inducible HeLa cell line. Pulse-chase analysis revealed that: i) the major secreted forms of PLTP carry complex N-glycans; ii) N-glycosylation is crucial for PLTP secretion; iii) Endo H-re...
متن کاملPig plasma phospholipid transfer protein facilitates HDL interconversion.
Phospholipid transfer protein (PLTP) from pig plasma was purified to homogeneity using ultracentrifugation and a combination of hydrophobic-, heparin-Sepharose-, and anti-pig-PLTP-affinity chromatography techniques. The molecular weight of PLTP is 78,000 as estimated by SDS-PAGE and by gel filtration. The effect of pig plasma PLTP on the particle size distribution of either human or pig high de...
متن کاملMeasurement of human plasma phospholipid transfer protein by sandwich ELISA.
BACKGROUND Plasma phospholipid transfer protein (PLTP) plays a central role in the remodeling of HDLs. Reliable and accurate methods for assaying PLTP concentration are required. METHODS A sandwich ELISA for PLTP has been developed, using two monoclonal antibodies against recombinant human PLTP (rhPLTP) expressed in Chinese hamster ovary cells. The ELISA allows for the quantification of PLTP ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.43.27393